Laboratory Manual For Practical Biochemistry (Paperback)

This book fulfills the need for a complete and comprehensive book on orientation towards various aspects of practical biochemistry. A clear-cut practical concept has been presented in respect of all qualitative and quantitative experiments. Chapters on demonstration experiments such as colorimetry, electrophoresis, chromatography etc. are also included. Questions at the end of each chapter and normal values of various biochemical parameters in blood and urine are incorporated. A concise, exam-oriented, knowledge-based laboratory manual for undergraduate students studying biochemistry.

Table Of Contents
Qualitative Experiment
1. Carbohydrates
2. Reactions of carbohydrates
3. Scheme for identification of unknown carbohydrates
4. Proteins
5.Color reactions of proteins
6. Precipitation reactions of proteins
7. Scheme for identification of unknown proteins
8. Reactions of non-protein nitrogenous substances
9. Scheme for identification of physiologically important substances
10. Urine analysis (Normal urine)
11. Analysis of abnormal constituents in urine
12. Analysis of gastric juice
13. Analysis of saliva

Quantitative Experiment
14. Sample collection and preservation
15. Estimation of blood sugar by Folin-Wu method
16. Estimation of blood glucose by O-toluidine method
17. Estimation of blood urea by diacetyl monoxime (DAM) method
18. Estimation of serum proteins and albumin/globulin ratio by Biuret method
19. Estimation of serum albumin by Dye-binding method
20. Estimation of serum inorganic phosphate by Fiske-Subba Row method
21. Estimation of serum cholesterol by Zak?s method

Laboratory Manual for Practical Biochemistry
22. Estimation of serum uric acid by Caraway method
23. Estimation of serum transaminases by Reitaman and Frankel method
24. Estimation of serum bilirubin by Malloy-Evelyn method
25. Estimation of serum creatinine by Jaffe?s alkaline picrate method
26. Estimation of urine creatinine by Jaffe?s alkaline picrate method

Demonstration Experiment
27. Colorimetry ? general principle and procedure
28. Electrophoresis
29. Chromatography
30. Preparation of hemin crystals

Oral Glucose Tolerance Test (ORAL GTT)
Case Reports
Normal Values Of Biochemical Parameters
Index