Concise, yet comprehensive text and easy to comprehend presentation. Simple and easy methods selected; all exercises tested before inclusion in the book. Comprehensively meets the requirements of undergraduate medical students. Suitable for dental/nursing/pharmacy and paramedical courses also. Principles and applications of analytical techniques like spectrophotometry, flame photometry, chromatography, and electrophoresis explained with the help of illustrations. Comprehensive discussion of some important topics like gastric analysis and urine examination Apart from updating existing chapters, two new chapters on "Radioimmunoassay" and "Enzyme-linked Immunosorbent Assay (ELISA) have been added in the revised second edition.
About the Author Dr. D.C. Sharma has 45 years of experience of teaching biochemistry, mainly to MBBS students and Biochemistry Postgraduates. He has taught in various Government Medical Colleges of Rajasthan, Himachal Pradesh and Haryana as Professor and Head of Biochemistry Departments. Presently, he is heading the Department of Biochemistry at S.G.T. Dental College, Budhera, Gurgaon. He has worked as a Post-Doctoral Fellow in Australia for 2 years. He has published 76 research papers in Indian and International journals, written 6 books in biochemistry and contributed chapters in two books.
Manminder Riyat had a brilliant career in Biochemistry at the Postgraduation level. She has taught Biochemistry to BDS students in S.G.T. Dental College, Budhera, Gurgaon, for 3 years, - where she published five books and six research papers as a coauthor of Dr. Sharma.
Table of Contents * Preface to the Second Edition * Preface to the First Edition * Section A: Qualitative Analysis * 1. Carbohydrates * 2. Proteins * 3. Lipids * 4. Food Stuff Milk, Eggs, Wheat Flour, Bread * 5. Urine * 6. Gastric Analysis * Section B: Quantitative Analysis * 7. Urinary Sugar * 8. Urinary Ascorbic Acid * 9. Urinary Ammonia * 10. Free and Total Acidities of Gastric Juice * 11. Total Chlorides of Gastric Juice * Section C: Analytical Techniques * 12. Colorimetry and Spectrophotometry * 13. Flame Photometry * 14. Chromatography * 15. Electrophoresis * Section D: Colorimetric Analysis * 16. Blood Glucose * 17. Urea * 18. Uric Acid * 19. Creatinine * 20. Cholesterol * 21. Calcium * 22. Inorganic Phosphorus * 23. Bilirubin * 24. Protein, Albumin and A-G Ratio * 25. Amylase * 26. Alkaline Phosphatase * 27. Acid Phosphatase * 28. Glutamate Oxaloacetate Transaminase * 29. Glutamate Pyruvate Transaminase * Section E: Immunologic Analysis * 30. Radioimmunoassay (RIA) * 31. Enzyme-linked Immunosorbent Assay (ELISA) * Appendix 1. Standard Reagents * Appendix 2. Normal Values of Biochemical Parameters * Appendix 3. Relation between Transmittance and Optical Density * Index
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