Genetic Engineering (Paperback)

Genetic Engineering is designed to serve as a textbook for the students of biotechnology, life sciences, genetics, microbiology, biochemistry, and other related areas. Replete with comprehensive details, it will also serve as a reference for the research students in this field.

Divided into four parts, the book provides in-depth coverage of all major topics in this area. The first part on fundamentals of genetic engineering includes concepts, such as gene cloning, enzymes used in genetic engineering, synthesis of nucleic acids, and techniques like gel electrophoresis and polymerase chain reaction.

The second part on vectors used in gene cloning covers the basic biology of plasmids, ? phages, other cloning vectors, and the strategies adopted for their application in gene cloning.

In the third part, generation and screening of recombinants are described. This part includes detailed discussions on DNA ligation, DNA delivery methods, construction of genomic and cDNA libraries, and screening of transformants.

The fourth part on applied genetic engineering covers DNA and protein sequencing, molecular markers, gene silencing, antisense RNA technology, and in vitro mutagenesis. Safety regulations related to genetically modified organisms (GMOs) are also discussed.

Written in a lucid style, the book is highly interactive, which will help readers understand the contents through visualization of concepts and techniques, and their practice.

Key Features
* Gives an elaborative account of the basic as well as the applied aspect of genetic engineering
* Provides an overview of recombinant DNA biosafety guidelines and National Regulatory Mechanism for their implementation
* Contains detailed illustrations, self-explanatory figures, and elaborate tables for easy understanding
* Includes numerous review questions for practice
* Contains exhibits with interesting facts along with web links

Table of Contents
Preface
List of Abbreviations
PART I: FUNDAMENTALS OF GENETIC ENGINEERING
1. An Introduction to Gene Cloning
2. Enzymes Used in Genetic Engineering
3. Restriction Endonucleases
4. Chemical Synthesis of Oligonucleotides
5. Synthesis of Complementary DNA
6. Techniques for Nucleic Acid Analysis and Size Fractionation
7. Polymerase Chain Reaction

PART II : VECTORS USED IN GENE CLONING
8. Plasmids
9. Bacteriophage
10. M13 Bacteriophage
11. Yeast Cloning Vectors
12. Vectors Other Than E. coli Plasmids, A. Bacteriophage, and M13

PART III : GENERATION AND SCREENING OF RECOMBINANTS
13. Joining of DNA Fragments
14. Introduction of DNA into Host Cells
15. Construction of Genomic and cDNA Libraries
16. Techniques for Selection, Screening, and Characterization of Transformants

PART IV: APPLIED GENETIC ENGINEERING
17. Other Techniques for Genetic Manipulation
18. Molecular Markers
19. Techniques Used in Genomics and Proteomics
20. Applications of Cloning and Gene Transfer Technology
21. Safety Regulations Related to Genetic Engineering

Glossary
Index

Table of Contents
Preface
List of Abbreviations

PART I: FUNDAMENTALS OF GENETIC ENGINEERING
Chapter 1. An Introduction to Gene Cloning
Chapter 2. Enzymes Used in Genetic Engineering
Chapter 3. Restriction Endonucleases
Chapter 4. Chemical Synthesis of Oligonucleotides
Chapter 5. Synthesis of Complementary DNA
Chapter 6. Techniques for Nucleic Acid Analysis and Size Fractionation
Chapter 7. Polymerase Chain Reaction

PART II : VECTORS USED IN GENE CLONING
Chapter 8. Plasmids
Chapter 9. Bacteriophage
Chapter 10. M13 Bacteriophage
Chapter 11. Yeast Cloning Vectors
Chapter 12. Vectors Other Than E. coli Plasmids, A. Bacteriophage, and M13

PART III : GENERATION AND SCREENING OF RECOMBINANTS
Chapter 13. Joining of DNA Fragments
Chapter 14. Introduction of DNA into Host Cells
Chapter 15. Construction of Genomic and cDNA Libraries
Chapter 16. Techniques for Selection, Screening, and Characterization of Transformants

PART IV: APPLIED GENETIC ENGINEERING
Chapter 17. Other Techniques for Genetic Manipulation
Chapter 18. Molecular Markers
Chapter 19. Techniques Used in Genomics and Proteomics
Chapter 20. Applications of Cloning and Gene Transfer Technology
Chapter 21. Safety Regulations Related to Genetic Engineering
Glossary
Index